ASTM E2562-2007 用疾病控制中心(CDC)生物膜反应器量化随高剪切连续流动生长的铜绿假单胞菌生物膜的标准试验方法
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【英文标准名称】:StandardTestMethodforQuantificationofPseudomonasaeruginosaBiofilmGrownwithHighShearandContinuousFlowusingCDCBiofilmReactor
【原文标准名称】:用疾病控制中心(CDC)生物膜反应器量化随高剪切连续流动生长的铜绿假单胞菌生物膜的标准试验方法
【标准号】:ASTME2562-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:连续的;流动;微生物学;假单胞菌;量化;反应器;剪切;测试
【英文主题词】:biofilm;reactor;growthreactor;shear;coupon;Pseudomonasaeruginosa;sampling
【摘要】:Bacteriathatexistinbiofilmarephenotypicallydifferentfromsuspendedcellsofthesamegenotype.Researchhasshownthatbiofilmbacteriaaremoredifficulttokillthansuspendedbacteria(5).Laboratorybiofilmsareengineeredingrowthreactorsdesignedtoproduceaspecificbiofilmtype.Alteringsystemparameterswillcorrespondinglyresultinachangeinthebiofilm.Forexample,researchhasshownthatbiofilmgrownunderhighshearismoredifficulttokillthanbiofilmgrownunderlowshear(6).ThepurposeofthistestmethodistodirectauserinthelaboratorystudyofaPseudomonasaeruginosabiofilmbyclearlydefiningeachsystemparameter.Thistestmethodwillenableaninvestigatortogrow,sample,andanalyzeaPseudomonasaeruginosabiofilmgrownunderhighshear.ThebiofilmgeneratedintheCDCbiofilmreactorisalsosuitableforefficacytesting.Afterthe48hgrowthphaseiscomplete,theusermayaddthetreatmentinsituorharvestthecouponsandtreatthemindividually.1.1ThistestmethodspecifiestheoperationalparametersrequiredtogrowarepeatablePseudomonasaeruginosabiofilmunderhighshear(1).Theresultingbiofilmisrepresentativeofgeneralizedsituationswherebiofilmexistsunderhighshearratherthanrepresentativeofoneparticularenvironment.1.2ThistestmethodusestheCentersforDiseaseControlandPrevention(CDC)biofilmreactor.TheCDCbiofilmreactorisacontinuouslystirredflowreactorwithhighwallshear.AlthoughitwasoriginallydesignedtomodelapotablewatersystemfortheevaluationofLegionellapneumophila(2),thereactorisversatileandmayalsobeusedforgrowingand/orcharacterizingbiofilmofvaryingspecies(3and4).1.3Thistestmethoddescribeshowtosampleandanalyzebiofilmforviablecells.Biofilmpopulationdensityisrecordedaslogcolonyformingunitspersurfacearea.1.4Basicmicrobiologytrainingisrequiredtoperformthistestmethod.1.5ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareforinformationonly.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C04
【国际标准分类号】:07_100_01
【页数】:6P.;A4
【正文语种】:
【原文标准名称】:用疾病控制中心(CDC)生物膜反应器量化随高剪切连续流动生长的铜绿假单胞菌生物膜的标准试验方法
【标准号】:ASTME2562-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:连续的;流动;微生物学;假单胞菌;量化;反应器;剪切;测试
【英文主题词】:biofilm;reactor;growthreactor;shear;coupon;Pseudomonasaeruginosa;sampling
【摘要】:Bacteriathatexistinbiofilmarephenotypicallydifferentfromsuspendedcellsofthesamegenotype.Researchhasshownthatbiofilmbacteriaaremoredifficulttokillthansuspendedbacteria(5).Laboratorybiofilmsareengineeredingrowthreactorsdesignedtoproduceaspecificbiofilmtype.Alteringsystemparameterswillcorrespondinglyresultinachangeinthebiofilm.Forexample,researchhasshownthatbiofilmgrownunderhighshearismoredifficulttokillthanbiofilmgrownunderlowshear(6).ThepurposeofthistestmethodistodirectauserinthelaboratorystudyofaPseudomonasaeruginosabiofilmbyclearlydefiningeachsystemparameter.Thistestmethodwillenableaninvestigatortogrow,sample,andanalyzeaPseudomonasaeruginosabiofilmgrownunderhighshear.ThebiofilmgeneratedintheCDCbiofilmreactorisalsosuitableforefficacytesting.Afterthe48hgrowthphaseiscomplete,theusermayaddthetreatmentinsituorharvestthecouponsandtreatthemindividually.1.1ThistestmethodspecifiestheoperationalparametersrequiredtogrowarepeatablePseudomonasaeruginosabiofilmunderhighshear(1).Theresultingbiofilmisrepresentativeofgeneralizedsituationswherebiofilmexistsunderhighshearratherthanrepresentativeofoneparticularenvironment.1.2ThistestmethodusestheCentersforDiseaseControlandPrevention(CDC)biofilmreactor.TheCDCbiofilmreactorisacontinuouslystirredflowreactorwithhighwallshear.AlthoughitwasoriginallydesignedtomodelapotablewatersystemfortheevaluationofLegionellapneumophila(2),thereactorisversatileandmayalsobeusedforgrowingand/orcharacterizingbiofilmofvaryingspecies(3and4).1.3Thistestmethoddescribeshowtosampleandanalyzebiofilmforviablecells.Biofilmpopulationdensityisrecordedaslogcolonyformingunitspersurfacearea.1.4Basicmicrobiologytrainingisrequiredtoperformthistestmethod.1.5ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareforinformationonly.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C04
【国际标准分类号】:07_100_01
【页数】:6P.;A4
【正文语种】:
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